• About BioTra-LC Pro
  • Strength of BioTra-LC Pro
  • Characters
  • Working Technique
  • Configuration
  • Detectors
  • Parameters
  • Support
  • Applications
  • Softwares
  • Accessories And Consuamables

We Study the Customer need and expecation in Diagnostics and Research based orginaization, so ours company very much proudly launched Biotra-LC PRO. It's are most Top End Model and unique design with stablity performance, we can perform any clinical diagnostics tests depends on the various application. The determination of purines and pyrimidines in biofluids is useful for the clinical-biochemical characterization of acute and chronic pathological states that induce transient or permanent alterations of metabolism. In particular, the diagnosis of several inborn errors of metabolism (IEMs) is accomplished by the analysis of circulating and excreted purines and pyrimidines. It is certainly advantageous to simultaneously determine the full purine and pyrimidine profile, as well as to quantity other compounds of relevance (e.g., organic acids, amino acids, sugars) in various metabolic hereditary diseases, in order to screen for a large number of IEMs using a reliable and sensitive analytical method characterized by mild to moderate costs. Toward this end, we have developed an ion-pairing BIOTRA–LC Pro   method with diode array detection for the synchronous separation of several purines and pyrimidines. This method also allows the quantification of additional compounds such as N-acetylated amino acids and dicarboxylic acids, the concentrations of which are profoundly altered in different IEMs. The application of the method in the analysis of biological samples from patients with suspected purine and pyrimidine disorders is presented to illustrate its applicability for the clinical-biochemical diagnosis of IEM.

Biotra-LC Pro is most powerful and innovative system to analyze multiple parameters in medical diagnostic analysis. The system is capable to satisfy all needs of user on quantitative, qualitative analysis and researches. Wide array of applications is possible with Biotra LC,That same is Clinical Diagnostics, Neonatal Diagnostics, Toxicology, Biotechnology, Life Science, Forensic Science Drug Analysis and Poising analysis

Biotra-LC Pro is most power full and innovative system to analysis multiple parameters in medical diagnostics analysis . The system is capable to satisfy all needs of quantitative, qualitative analysis and research function according its design

  1. Most accurate technology for liquid kind of sample analysis
  2. Biotra-LC Pro  will be provide High separation capacity, enabling the batch analysis of multiple components
  3. WHO Approved Technology for  liquid kind of sample analysis
  4. Superior quantitative  and  qualitative  Analysis capability
  5. Moderated  analyzing  conditions
  6. One  Tool for Multiple Application
  7. Low sample consumption
  8. Easy preparative separation and purification of samples
  9. Easy sample preparation
  10. Less cost for sample analysis
  11. Suitable instrument for sample analysis and also research kind of analysis
  12. Excellent supporting for Academic projects
  13. Easy updatable and upgradable, no need for changing instrument, because its top most model.
  1. Excellent supporting for Academic projects
  2. Easy upgradable to Pro Version without changing instrument

Accurate: - Accuracy is the sprite of analysis ‘ Based on well design and manufacturing Process un questionable accuracy and precision were a prerequisite , secondly quantification and precision of analysis were dependent on accurate flow Rate.

Stability: - Excellent Reliability and stability. The pump heads have been machined using a first class CNC Process the innovative structural design has been designed to achieve Minimum pressure function. Consequently stable flow, Low noise and overall quality provide the foundation for reliable results.

Powerful: - Biogen Lc operational software offers complete control for Biotra Lc. Powerful data processing, Reporting Function, Intelligent Diagnostics Systems and Maintenance utility ensure a complete and flexible software solution for your analyzing Works.

Economical: - The Biotra – Lc is High Performance yet cost effective solution the modular design will ensures that you can configure the system to meet your Application requirements Even later we can easily upgrade the system without changing of your basic configuration. Detected team of product specialists will provide technical support and provide an application development services.

Biotra – LC Pro is a separation technique in which the mobile phase is an aqueous solution or an organic solvent that is miscible with water, and the compounds of interest are dissolved in water or an organic solvent such as methanol. The major advantage of the technique is the flexibility it allows in choosing the stationary and mobile phases that best separate the particular sample components. More than half of the types of columns used in Biotra – LC Pro   is reverse phase; around a quarter are normal phase; about 14% are ion-exchange columns. Obtaining high resolution at high flow rates is the key requirement of any Biotra – LC Pro   system. Separation of the different components of a mixture is achieved by allowing the sample to migrate through the column at selected experimental conditions. Discrete bands are formed because the different components of the mixture migrate through the column at different rates.

The apparatus of a typical liquid chromatography system includes an fluent system, a pump, an automatic sampler, the analytical column, a detector and a recorder. The most essential component of this apparatus is the analytical column that is used to separate the components of interest in biological samples. The most widely used detector for Biotra – LC Pro   is the UV-Visible and fluroscopy absorbance detector. This is a sensitive detector for organic molecules that absorb light within the UV or visible wavelength spectrum, which is widely applicable to many compounds. The difficulty often faced in analyzing clinical samples is resolving the compounds of interest from interfering substances, as well as the need to detect trace quantities of analytics. In addition to analyzing compounds which are the result of metabolism in both healthy and diseased states, Biotra – LC Pro   can also be used to measure
Therapeutic drug levels as well as the presence of drugs of abuse.

Good sample preparation is essential for the successful analysis of specimens, which include biological samples such as blood and urine. Blood in particular needs to be processed prior to analysis to remove any interfering compounds that would cause either contamination or blockage of the column, giving inaccurate results. These problems can be prevented with the use of a guard column with larger particle size packing material. Sample preparation also involves the typical methods of solvent extraction, ultra filtration or solid-phase extraction. With total automation, time spent on sample extraction and clean-up procedures are minimized, while the sensitivity and simplicity of the technique are maintained. This has proven extremely useful in the analysis of catecholamines and in determination of amino acid metabolism.

Biotra –LC Pro is offering various configurations so our clients can select models according their requirement without constrain. This compliance will took correct configuration without wasting Money and also can be upgrade at any time without changing of basic configuration.



An ELSD (EVAPORATIVE LIGHT-SCATTERING DEDECTOR) atomizes the column cluate, shines light on the resulting particulate components and detects the resulting scattered light. Theoretically, an ELSD can detect any nonvolatile component. An ELSD has sensitivity roughly 10 times higher than an RI detector, but has a slightly low sensitivity to low molecular components due to theor small size. An ELSD is used mainly to detect non-UV – absorbing components. Attention should be given to the fact that nonvolatile salts cannot be used as the fluent.


ELSD can outperform traditional detectors when analyzing non- chromophoric samples by HPLC.

Can detect anything that is less volatile than the mobile phase.

ELSD is universal and compatible with a wide range of solvents.

Wide gradient compatibility, no limitation on choice of solvents.

High sensitivity, extremely low limits of detection.

Wide application range to cover very low to high molecular weight compounds.

Low dispersion, minimal peak broadening for maximum resolution.

Rabid equilibration, fast set-up and high productivity.

Advanced, easy to use instrument control with built- in safety.

Optimum performance for all applications.


Fluorescence detectors are probably the most sensitive among the existing modern HPLC Detectors its possible to detect even a presence of a single Analytes molecule in the flow cell. Typically fluorescence sensitivity is 10- 1000 higher than that of the UV detector for strong UV absorbing materials. Fluorescence detectors are very specific and selective among the others optical detector. This is normally used as an advantage in the measurement of specific fluorescent species in samples

When components having specific functional groups are excited by shorter wavelength energy and emits Higher wavelength radiation which called fluorescence  usually The emission is measured at Right angles to the excitation fluorescence intensity depends on both the excitation and emission wavelength allowing selectively detect some components’ while suppressing the emission of others
The detection of any component significantly depends on the chosen wavelength and if one component could be detected at 280 ex and 340Em, another could be missed most of the modern detectors allow fast switch of the excitation and emission wavelength. Which offer the possibility to detect all components in the mixture For example in the very important polynuclear aromatic chromatogram the exceetitation and emission wavelengths were 280 and 340 nm, Respectively for the first 6 components’ and then changed to the respective values of 305 & 430 nm, The later values we represent the best compromise to allow sensitive detection of components’


Innovative axially illuminated flow cell designed for less desperation less stray light less volume and       more bath length for maximum sensitivity

Three dimensional spectral scanning   for faster method  development and optimization and enhanced  peak identification

Single or multi- channel operation monitors fluorescence at one or more discrete wavelength pairs 

Low noise performance – less stray Light due to or thogonal optics and the use of mirrors not lenses minimize scatter

Multiple detection Modes – 2D,3D . And on – the- fly . spectral scanning –can greatly reduce the time needed to develop fluorescence   detection methods by quickly determining wavelength maximum limit


Electrochemical detection delivers the high sensitivity needed for the measurement of neurotransmitters, the simplicity and robustness needed for pharmaceutical or clinical diagnostics , and the selectivity for characterization of complex samples  such as natural products, biological tissues, and fluids.
Analyze Biotech is Offering ECD-2000 electrochemical detector features all new electronics with advanced digital processing , guaranteeing  the lowest possible detection limits from micro bore to normal bore for both HPLC and  UHPLC. The high sensitivity and low noise of this electrochemical detector makes it ideal for the most sensitive and demanding analyses.


Intelligence chips will provides automatic instrument configuration with optimized control of an attached electrochemical sensor.
Fluidic components are exchangeable from the front of the detector without special tools.
Front – panel display provides easy monitoring of detector status to maximize up time.
Full scale signal auto ranging protects data integrity by preventing data loss to peak over ranging.
Gradient compatibility from the only electrochemical detector capable of working with aggressive gradients for method flexibility.
Plug and play electrochemical cells, which eliminate lengthy cable connections and noise.
Colorimetric and Amperometric designs.
Amperometric sensor available with a variety of working electrode materials, including boron – doped diamond.
HPLC AND UHPLC compatible.
Maintenance – free solid state reference electrodes.


Photo diode arrays (semiconductor device) are used in the detection unit. A DAD detectors the absorption in UV to VIS region. While a UV-VIS has only one sample –side Light receiving section a DAD has multiple (1024 for 102455/2455U) Photodiode arrays to obtain information over wide range of wavelengths at one time which is a merit of the DAD
The idea is that spectra are measured at intervals of 1 second or less during separation by Biotra – Lc with continuous evaluate delivery. If the measurement is performed at fixed wavelength. Components are identified from only their retention time thus , A minor deviation in retention time cane make identification of  components difficult in such a cause, the DAD can be used to identify components  by a compression of the spectrum.

DAD s differ from UV-VIS detectors in that light from the lamp is shown directly on to the flowcel , light that passes through the flowcel is dispersed by the diffraction grating , and the amount of the dispersed light is estimated for each wavelength in the photodiode arrays.


High Accuracy

High Speed scanning

Support to find Molecules

Up to 4 spectra’s can be saved

Analyze can be done while liquid moving


An RI detector detects components based on the refraction of light in solution. The flow of RI detectors is divided into the sample – side and reference –side cells. Prior to analysis, elute is introduced into either cell. Until the flow of the elute becomes equilibrated. The reference-side cell is filled with elute and the column elute is introduced into the sample-side cell through the changed flow channel. When components are eluted from the column the chemical composition changes in the sample-side solution. Which changes its photorefractive level as a result, the amount of high received by the light-receiving section changes, showing a peak which can be detected. Any component in the elute can be detected. Thus, the RI detector is often called a “universal detector”.


Detection of components without UV chromophoric separated by isocratic analysis.

Highly repeatable detection technique with a wide linear dynamic range for methods recurring quantification.

Thermally isolated optics bench for temperature stability and performance reproducibility.

Countercurrent heat exchanger to quickly equilibrate temperature differences of incoming solvent.

Save solvent and maintain stability with convenient recycle valve.

Auto –zero, auto-purge and LED-displayed diagnostic programming enable unattended operation and to simplify methods development.

Control of external column heater.



PDD has two different modes one is PDECD and another one PDHID. The PDECD is a selective detector for monitoring high electron affinity compounds such as Freon, chlorinated Pesticides and other halogen compounds for this type of compound the minimum detectable quantity (MDQ) is at the femtogram (10 – 15)   or pictogram (10-12) level. The PDD is similar in sensitivity and response characteristics to a conventional radioactive ECD and can be operated at temperature up to 400 c.

For operation in this mode He and CH4 are introduced just upstream from the column exit. The other mode PDHID is an universal non destructive high sensitivity detector. The response to both inorganic and organic compounds is linear over a wide range response to fixed gases is a positive (increasing in standing current) with an MDQ in the low PPb range.


High Accuracy
High Speed scanning
Analyze can be done while liquid moving

Highly repeatable detection technique with a wide linear dynamic range for methods recurring quantification.
Thermally isolated optics bench for temperature stability and performance reproducibility.


Flame Ionization detector (FID)

For detection of any organic components which can be ionized by hydrogen / air flame

Maximum Temperature  450 c

Automatic flame Ignition  /Flame out detection

MDL: < 3.2 Pg Carbon / sec as doclecane using N2  carrier and 0.4572 mm jet

Linear dynamic range <± 10%, 10 7 with N2 carrier and 0.4572mm jet

Sensitivity : 19m coulombs/sec



Biotra – LC PRO will support to produce results on qualitatitive and quantitative form with High accuracy, The output results we can carry to clinical studies and research procedures. Biotra – LC PRO never think about compromise on its Out Put.
Biotra – LC PRO and its consumables is specially designed for clinical Diagnostics analysis and research and also much medical applications and Research Oriented Work

Online Service Support

Analyze Biotech Is Offers Completely Online Service Support To High Cost Instruments So Analyze Biotech Is Reducing Down Time Of The Instruments.

Onsite Support

Analyze Biotech Is Loaded With Highly Trained Professionals To Provide Engineering Support And Application To Ours Valuable Clients. Our Supporting Team Peoples Will Be Give Excellent Working Nature So Our Clients Can Enjoy Presence Of Our Professionals. We Are Have Clear Supply Chain For Spare Parts And Consumables So Will Ensuring Trouble Free Performance Module For Your Equipments

Application Support

Customers Are Different Same Their Requirements Also Different, So We Clearly Understanding This Factor So Analyze Biotech Is Bringing Unique Application Concept For Each And Every Customers Based On Their Requirement . Our Customer Based Concentration Will Bring Excellent Fortune To Our Clients. The Customers Can Bring Their Queries Through Our Website These Queries Will Be Revert To The Corresponding Department And Addressed Within No Time.

Upgrade Support

Analyzing Biotech Has Various Upgrade Solutions As An Added-Value Service To Meet Customer’s Requirements For Function, Application Or Performance. *Upgrade May Not Be Applicable For Some Models.

Validation Support

As Per Who Norms Each And Every Instruments Should Undergone Validation Function For Cycle Of Period Which Is Ensuring System Performance Up To Standard So Ab Is Moving To Take Kind Of Task To Ensure All Our Customers Systems To Stay On This Standard Up To Life Time Of The Equipment

Certification Support

Certifications Are Most Powerful Public Key To Establish Our Reputation. Analyze Biotech Will Provide Excellent Documentation Support To Bring Your Certifications Dream Like Nabl, Nhbl Standards Come True.

Biotra –LC PRO is Most Powerful and innovative system to analyze multiple Parameters in Medical Diagnostics analysis and also the system is capable to analyze Many Kind of research functional areas. Biotra –LC PRO satisfy all needs of quantitative, qualitative analysis and research function in Medical Applications 
Biotra –LC PRO'S Various Functional Approaches 

  1. Clinical Diagnostics
  2. Neonatal Diagnostics
  3. Toxicology
  4. Biotechnology
  5. Life Science
  6. Forensic Science
  7. Drug Analysis
  8. Poison Analysis 

Biotra –LC PRO is offering wide array of detectors to achieve wide array of applications without compromise so Biotra-Lc users need not to worry about constrains of analysis. We cared users constrains and Limitations while Manufacturing of Biotra –LC PRO
Biotra –LC PROand its accessories are specially designed to meet all medical applications with single tool. The results are more accurate compare with other Technologies.Biotra –LC PRO considered sensitive with high working Module

Strength of Biotra –LC PRO

  1. Biotra – LC Pro will be provide High separation capacity enabling the batch analysis of multiple components .
  2. WHO approved Technology for liquid kind of sample analysis.
  3. Superior quantitative and qualitative analysis capability .
  4. Moderated analyzing Conditions .
  5. One tool for Multiple applications .
  6. Low sample consumption .
  7. Easy preparative separation and purifications of samples .
  8. Analyzing Main parameters including its sub parameters .
  9. New methods developments are easy .
  10. Unknown Samples Measurement .
  11. Versatile detectors can be attached for Multiple application.
  12. Same system can be  update without changing of Main units.
  13. Easy system adaptability for other samples like Oil, Water and food and beverages so many sample analysis possible for Research and analysis .


BioGen is a most powerful software tool to operate Biotra – Lc and its variants. The software is designed to easy operation
The Software Module will build with the functions of

  1. Complete system control
  2. Online monitoring
  3. Hardware Management
  4. Up gradation   computability
  5. Remote support and service
  6. Operation  & Data sharing Over networking
  7. Data storage
  8. Multi application support
  9. Suitable for complete Biotra – Lc variants


Analyze Biotech, offer all the Consumables / Reagents / Quality Control Standards / Calibration Sandards / required for running the Analyzers like BIOTRA-LC PRO.

BIOTRA-LC PRO, Consumables like

Valves, Plunger (Piston) ,

Plunger Seals,

Line Filters,

Pro-Suction Filters,

LCRotor Seals

Pulse Damper,

Auto-Mixer, Autosampler vials,

Biotra-LC PRO-Reagent kits and Solvent,

Biotra-LC PROColumns

Mesh-damper proof cartridge, makes routine maintenance fast, realiablity, and stablity
Pistons are high-tech engineered to insure a high quality surface finish
Packed-Seals are designed for long life, multi range of solvent compatibility, and optimum in high performance
Auto Check Valves, result best performance

Source Lamps :

Lamps for BIOTRA-LC PRO, such as D2, Halogen, Xenon. Tungsten, Mercury vapors, Hallow Cathod Lamps ,etc,.